Authors:

Hee Wan Kang

Abstract:

“Twenty primers of 20 mer referred to universal rice primer (URP) were developed from a repetitive sequence of rice genome. URP-PCR protocol employed stringent PCR with high annealing temperature throughout the thermo-cycling reaction, giving high reproducibility. Under the PCR condition, each single URP primer produced characteristic fingerprints from diverse genomes of bacterial species. The universal application of URP-PCR was demonstrated by applying it to 24 strains from Pectobacterium carotovoum subsp. carotovorum, 41 Agrobacterium vitis strains, 3 Xanthomonas spp. 5 Pseudomonas spp, Rhizobium sp. plant pathogenic bacteria, human and animal pathogenic bacterial strains including 6 Escherichia coli, 4 Salmonella spp., 7 Mycobacterium spp and 3 Blucella abortus strains. In addition, thermophilic bacteria were randomly isolated form high temperature compost and their URP-PCR polymorphisms were characterized with genetic relatedness. PCR approach using URP primers will be useful for studying DNA diversity of diverse prokaryotic genomes, especially at inter- and intra species levels.”

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PDF:

https://jurnal.harianregional.com/jbb/full-44266

Published

2018-12-04

How To Cite

KANG, Hee Wan. PCR FINGERPRINTING OF DIVERSE GENOMES FROM BACTERIAL STRAINS USING UNIVERSAL RICE PRIMER (URP).International Journal of Biosciences and Biotechnology, [S.l.], v. 6, n. 1, p. 51-64, dec. 2018. ISSN 2655-9994. Available at: https://jurnal.harianregional.com/jbb/id-44266. Date accessed: 08 Jul. 2024. doi:https://doi.org/10.24843/IJBB.2018.v06.i01.p06.

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Issue

Vol 6 No 1 (2018)

Section

Articles

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