Authors:

Byoung Ho So, Jongun Kim, Hee Wan Kang

Abstract:

“Thirty-mer oligonucleotides for microarray analysis were designed from the annotated open reading frames(ORFs) in the whole genome sequence of X. oryzae pv. oryzae KACC10331. Mutant hrpXo::TN and wild-typestrain KACC10859 were cultured in hrp inducing medium (XOM2), and cDNAs, which were synthesizedfrom the total RNA samples from both strains, were hybridized on a DNA microarray. The microarraydata showed that 210 genes were down-regulated more than 2-fold in hrpXo::TN, while 115 genes wereup-regulated more than 2-fold. The HrpXo regulons diff erently included 54 hypothetical genes: type IIIsecretory genes (11 hrp genes); genes encoding type III secretory eff ectors (xopP genes and avr/pthA); genesencoding type II secretory eff ectors (6 proteases, a lipase, a polygalacturonase, and 4 cellulases); 7 iron-uptakegenes; 6 pil genes encoding fi mbrial assembly membrane proteins; and 14 transposon genes. Signifi cantplant-inducible promoter (PIP) sequences were newly identifi ed on HrpXo regulons. The validity of theexpressional profi les was further confi rmed by reverse transcription (RT)-PCR.”

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PDF:

https://jurnal.harianregional.com/jbb/full-13592

Published

2021-11-09

How To Cite

SO, Byoung Ho; KIM, Jongun; KANG, Hee Wan. DNA MICROARRAY BASED EXPRESSIONAL PROFILING OF HRPXO DEPENDENT UP-REGULONS IN XANTHOMONAS ORYZAE PV. ORYZAE.International Journal of Biosciences and Biotechnology, [S.l.], v. 1, n. 1, july 2013. ISSN 2655-9994. Available at: https://jurnal.harianregional.com/jbb/id-13592. Date accessed: 28 Aug. 2025.

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Issue

Vol 1 No 1 (2012)

Section

Articles

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