Authors:

Vanesa Martida, Made Pharmawati

Abstract:

“There are many variation of Plumeria sp. that grown in Bali. The genetic identity of Plumeria sp. need to be analysedusing molecular study for plant breeding purpose. DNA extraction and primer selection are basic steps for molecular studyespecially in identification and analysis of genetic diversity. The aim of this research was to determine RAPD primerssuitable for molecular analysis of Plumeria sp. This research used CTAB method with modification for DNA extraction. Thesamples were young leaves of Plumeria sp. dried using silica gel. The primers used were produced by University of BritishColumbia and Operon Primer Technology. The results showed that DNA concentration of Plumeria sp from dried leaves wasbetween 33-267 ng/?l. Out of seven primers tested, three primers UBC-127, UBC-250, and OPH-06 produced clear andscorable amplification products for further analyses.Keywords: DNA, Plumeria sp., RAPD primer”

Keywords

DNA, Plumeria sp., RAPD primer

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PDF:

https://jurnal.harianregional.com/simbiosis/full-21226

Published

2021-11-09

How To Cite

MARTIDA, Vanesa; PHARMAWATI, Made. PEMILIHAN PRIMER RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) PADA PCR (POLYMERASE CHAIN REACTION) TANAMAN KAMBOJA (Plumeria sp.).SIMBIOSIS, [S.l.], apr. 2016. ISSN 2656-7784. Available at: https://jurnal.harianregional.com/simbiosis/id-21226. Date accessed: 02 Jun. 2025.

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Issue

Vol 4, No 1 (2016)

Section

Articles

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License