Authors:

Ni Wayan Tianing

Abstract:

“ND3 gene is a mitochondria DNA gene which code for NADH dehydrogenase enzime, part of complex 1 respiratory chain oxidation and involved in phosphorilation prosess to synthesize ATP (Adenosin Triphosphat). The present of G10398A variant at mtDNA result in changing the amino acid code for alanine (GCC) to threonine (ACC). This changing induce elevation the ROS (Reactive Oxygen Species) concentration in mitochondria. ROS can cause oxidation of DNA, mutation of DNA,oxidative stress and cancer. The other effect of ROS can affect redox-cycling estrogen metabolism. Individual with G10398A variant has 60% risk for breast cancer. The purpose of this research are to identify G10398A variant and its proportion in ND3 gene with non experimental descriptive method within 32 samples and 5 control. This research has done with sequence of steps with an isolation and total DNA amplification electrophoresis, sequencing and analysis by comparing with gene bank. This research identify G10399A variant that causes changing code from serine (AGC) amino acid in to asparagines (AAC) in 16 samples. Another variant found in this research is C10401T variant the cause changing in codon that code from arginin (CGA) in to triptopan (TGA) within 14 samples. In the control groups 2 samples identify with G10399A variant, 2 samples with C10401T and 1 control match like gene-bank. For this research, ND3 gene homology that was amplified found in the average of 96%.”

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PDF:

https://jurnal.harianregional.com/medicina/full-5068

Published

2021-11-09

How To Cite

TIANING, Ni Wayan. IDENTIFICATION OF G10398A VARIAN OF ND3 GENE MITHOCONDRIA DNA IN BREAST CANCER PATIENT.Medicina, [S.l.], v. 43, n. 3, apr. 2013. ISSN 2540-8321. Available at: https://jurnal.harianregional.com/medicina/id-5068. Date accessed: 28 Aug. 2025.

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Issue

Vol 43 No 3 (2012): September 2012

Section

Articles

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License