This study aims to determine the genetic character of cyprinid fish Barbonymus sp using the mtDNA gene in the COI and Cytb gene regions Samples were collected from the various areas in East Java Province including Gresik Mojokerto Madiun Malang and Banyuwangi The caudal peduncle of the cyprinid fish was used for molecular identification Isolation and purification of its genomic DNA using the gSYNCTM DNA Extraction kit We used a polymerase chain reactio

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