Authors:

Jaya, I. G. N. I. P, Leliqia N. P. E, I.N.K. Widjaja

Abstract:

“The purpose of this research was to compare the DPPH scavenging activity of black tea (Camellia sinensis (L.) OK) and gambier (Uncaria gambir (Hunter) Roxb) product extract and to determine their TLC-densitometer profile. This research was conducted in two stages, first stage was to examine the DPPH scavenging activity of black tea and gambier product extract. This assay used some concentrations of black tea product extract, gambier product extract, and standard vitamin C (10, 20, 40, 80, 160, 200, and 320 µg/mL). The second stage was to determine the extracts profile by TLC-densitometer. The result of this research showed that the DPPH scavenging activity of gambier product extract was greater (IC50 = 88,57±0,64 µg/mL) than black tea product extract (IC50 = 311,54±2,79 µg/mL). The result of profile determination of black tea product extract at a mobile phase of MeOH:toluene (50:50) showed that no spot was supposed a volatile oil group; at mobile phase of ethyl acetate:formic acid:acetic acid:H2O (100:11:11:26) showed two spots that was supposed a flavonoid group, spot with Rf 0,41 (?max 365 nm) and spot with Rf 0,97 (?max 280 nm); at mobile phase of CHCl3:MeOH (90:10) showed that spot with Rf 0,87 (?max 275 nm) was supposed an alkaloid group; at mobile phase of acetic acid:diethyl ether:n-hexane:ethyl acetate (20:20:20:40) showed that spot with Rf 0,62 (?max 290 nm) was supposed a tannin group. While the result of profile determination of gambier product extract at mobile phase of ethyl acetate:formic acid:acetic acid:H2O (100:11:11:26) showed two spots that was supposed a flavonoid group, spot with Rf 0,81 (?max 280 nm) and spot with Rf 0,92 (?max 280 nm); at mobile phase of acetic acid:diethyl ether:n-hexane:ethyl acetate (20:20:20:40) showed that spot with Rf 0,42 (?max 285 nm) was supposed a tannin group.”

Keywords

Keyword Not Available

Downloads:

Download data is not yet available.

References

References Not Available

PDF:

https://jurnal.harianregional.com/jfu/full-5194

Published

2021-11-09

How To Cite

I. G. N. I. P, Jaya,; N. P. E, Leliqia; WIDJAJA, I.N.K.. UJI AKTIVITAS PENANGKAPAN RADIKAL DPPH EKSTRAK PRODUK TEH HITAM (Camellia sinensis (L.) O.K.) DAN GAMBIR (Uncaria gambir (Hunter) Roxb) SERTA PROFIL KLT-DENSITOMETERNYA.Jurnal Farmasi Udayana, [S.l.], mar. 2013. ISSN 2622-4607. Available at: https://jurnal.harianregional.com/jfu/id-5194. Date accessed: 28 Aug. 2025.

Citation Format

ABNT, APA, BibTeX, CBE, EndNote - EndNote format (Macintosh & Windows), MLA, ProCite - RIS format (Macintosh & Windows), RefWorks, Reference Manager - RIS format (Windows only), Turabian

Issue

Vol. 1, No. 1, Tahun 2012

Section

Articles

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License