Authors:

Pande Made Nova Armita Sari, Ni Putu Eka Leliqia

Abstract:

“A current research of screening and determination of codeine using Al-TLC Spectrophotodensitometry has been carried out. The separation was performed using TAEA mobile phase. Screening and confirmation were investigated by comparing hRfc of the analyte and hRfc those on data library. Combination hRfc vs correlation also been used as confirmation method, which can be enhanced by spectrum shift reaction using HCl 10% and KOH 0,1 M. The validation parameters were precission, linierity, limit of detection and quantitation (LOD & LOQ) and recovery. TAEA mobile phase performed a good separation among morphine, codeine, caffeine, papaverine and bromhexine standards, which gave Rs value > 1. Test of linierity using regression gave correlation value > 0,99 . LOD and LOQ of Al-TLC were 137.85 ng and 459.63 ng. The recovery of codeine in human plasma using isopropanol as the precipitating agent was 77.1% ± 4.9 (6.5%).”

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PDF:

https://jurnal.harianregional.com/ijlfs/full-20897

Published

2013-05-08

How To Cite

ARMITA SARI, Pande Made Nova; LELIQIA, Ni Putu Eka. UJI SKRINING DAN DETERMINASI KODEIN DENGAN TLC SPEKTROFOTODENSITOMETRI.Indonesian Journal of Legal and Forensic Sciences (IJLFS), [S.l.], v. 3, may 2013. ISSN 2657-0815. Available at: https://jurnal.harianregional.com/ijlfs/id-20897. Date accessed: 28 Aug. 2025.

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Issue

Vol 3 (2013): Indonesian Journal of Legal and Forensic Sciences

Section

Articles

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