Authors:

I Made Merdana, Ida Ayu Pasti Hapsari, Fuady Muslih

Abstract:

“The Neem leaves were very potential to developed as an anti-ectoparasite drug in pets. The purpose of this research was to determine the effectiveness of ethanol extract of Neem leaves (Azadirachta indica, A. Juss) against Rhipicephalus sanguineus dog by in vitro. Using a completely randomized design, 250 ticks were randomly divided into 5 groups with five repetitions. A gentle spray treatment of neem leaves extract, sequentially; P0 as negative control with Aquadest, P1 as positive control with 0.5% deltamethrin, P2 with 2.5% Neem leaves extract, P3 with 5% Neem leaves extract, P4 with 10% Neem leaves extract, Each group consists of 12 ticks that placed in petri dish with a cotton pad that has been sprayed with each solvent, then treated again by spraying it gently and equal. The results of the research showed that the ethanol extract of neem leaves proved to be very significant (P <0.01) against the mortality of R. sanguineus by in vitro. This research, solvent with 10% of neem leaves extract was most effective to exterminate R. sanguineus ticks with 100% mortality.”

Keywords

Keyword Not Available

Downloads:

Download data is not yet available.

References

  • Bukhari. 2011. Efektifitas ekstra daun mimba terhadap pengendalian hama Plutella xylostella L. pada tanaman kedele. J. Sains Riset. 1(1): 11.
  • Hadi UK, Soviana S. 2015. Prevalence of ticks and tick-borne diseases in Indonesian dogs. J. Vet. Sci. Technol. 07(3): 15-20.
  • Hutasoit IH, Siswanto, Merdana IM. 2015. Uji efektivitas ekstrak akar tuba (Derris elliptica) terhadap caplak anjing secara in vitro. Indonesia Mediscus Veterinus. 4(2): 122-128.
  • Javandira C, Widnyana IK, Suryadarmawan IGA. 2016. Kajian fitokimia dan potensi ekstrak daun tanaman mimba (Azadirachta indica, A. Juss) sebagai pestisida nabati. Prosiding Seminar Nasional. Unmas Denpasar. Pp: 402-406.
  • Katzung BG. 2004. Basic and Clinical Pharmacology. 9th. The McGraw-Hill Companies. United State.
  • Levine ND. 1994. Buku Ajar Parasitologi Veteriner. Yogyakarta. Terjemahan: G. Ashandi. Gadjah Mada University Press.
  • Mardiningsih TL, Sukmana C, Tarigan N, Surati S. 2010. Efektivitas insektisida nabati berbahan aktif azadirachtin dan saponin terhadap mortalitas dan itensitas serangan aphis gossypii glover. Buletin Littro. 21(2): 171-183.
  • Matsumura F. 1985. Tocxicology of Pesticides. Plenum Press. New York. Pp: 17-22.
  • Rani PAMA, Irwin PJ, Coleman GT, Gatne M, Traub RJ. 2011. A survey of canine tick-borne diseases in India. Parasites Vectors. 4: 141.
  • Roma GC, Mathias MIC, Oliveira PR, Furquim KCS, Bechara GH. 2013. Neurotoxic action of permethrin in Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) female ticks; Morphological and cytochemical evaluation of the central nervous system. Vet. Parasitol. 196(3): 482–491.
  • Samsudin. 2011. Biosintesa dan cara kerja azadirachtin sebagai bahan aktif insektisida nabati. Prosiding Seminar Nasional Pestisida Nabati IV. Jakarta.
  • Setiawan PH, Siswanto, Merdana IM. 2014. Ekstrak akar tuba (Derris elliptica) efektif membunuh pinjal (Siphonaptera) kucing secara in vitro. Indonesia Mediscus Veterinus. 3(5): 323-429.
  • Steel RGD, Torrie JH. 1993. Principles and Procedurs of Statistics A. Biometrical Approach. 2nd Ed. New York: McGraw-Hill. Kogakusha Ltd.
  • Stenersen J. 2004. Chemical Pesticides Mode of Action and Toxicology. CRC Press.
  • Wirawan IGKO, Jadi ML, Hadisutanto B. 2010. Efek ekstrak mimba sereh dan lengkuas terhadap infestasi caplak pada anjing. Patner. 1: 37-42.
  • Whiteford F, Fuhremann T, Rao KS, Klaunig JE. 2017. Pesticide Toxicology; Evaluating Safety and Risk. Purdue University. Cooperative Extension Service. West Lafayette.
  • Zhang QW, Lin LG, Ye WC. 2018. Techniques for extraction and isolation of natural products: A comprehensive review. Chin. Med. 13: 20.

PDF:

https://jurnal.harianregional.com/buletinvet/full-56278

Published

2020-02-28

How To Cite

MERDANA, I Made; HAPSARI, Ida Ayu Pasti; MUSLIH, Fuady. THE EFFECTIVENESS OF NEEM LEAF ETHANOL EXTRACT ON THE IN VITRO OF RHIPICHEPALUS SANGUINEUS.Buletin Veteriner Udayana, [S.l.], p. 86-91, feb. 2020. ISSN 2477-2712. Available at: https://jurnal.harianregional.com/buletinvet/id-56278. Date accessed: 02 Jun. 2025. doi:https://doi.org/10.24843/bulvet.2020.v12.i01.p15.

Citation Format

ABNT, APA, BibTeX, CBE, EndNote - EndNote format (Macintosh & Windows), MLA, ProCite - RIS format (Macintosh & Windows), RefWorks, Reference Manager - RIS format (Windows only), Turabian

Issue

Vol. 12 No. 1 Pebruari 2020

Section

Articles

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License