Authors:

Zefarina Zulkafli, Rapiaah Mustaffa, Shafini Mohammed Yusoff

Abstract:

“Background: To date, the CD34+ cell enumeration has relied predominantly on flow cytometry technique. However, flow cytometry is time consuming and operator dependent. The application of the hematopoietic progenitor cells (HPCs) channel in Sysmex XE-2100, a fully automated hematology analyzer offers an alternative approach, which is with minimal sample manipulation and less operator dependent. This study evaluates the utility of HPC counts as a predictive of CD34+ enumeration prior to peripheral blood stem cells harvesting. Materials and methods: HPC, CD34+, white blood cell (WBC), reticulocytes (retic), immature platelet fraction (IPF) and immature reticulocyte fraction (IRF) were determined in 61 samples from 19 patients with hematological malignancies (15 lymphoma and 4 multiple myeloma patients) at Hospital Universiti Sains Malaysia (Hospital USM) who had received granulocyte-colony stimulating factor (G-CSF) and planned for autologous transplantation. Results: CD34+ count showed strong and significant correlation with HPC. The receiver operating characteristics (ROC) curve analysis revealed that HPC count > 21.5 x 106 / L can predicts a pre harvest CD34+ count of >20 x 106 / L with sensitivity of 77%, specificity of 64% and area under the curve (AUC) of 0.802. Conclusion: We concluded that HPC count can be a useful potential parameter in optimizing timing for CD34+ enumeration prior to leukapheresis.”

Keywords

Keyword Not Available

Downloads:

Download data is not yet available.

References

References Not Available

PDF:

https://jurnal.harianregional.com/bmj/full-21604

Published

2014-09-04

How To Cite

ZULKAFLI, Zefarina; MUSTAFFA, Rapiaah; YUSOFF, Shafini Mohammed. HEMATOPOIETIC PROGENITOR CELLS AS A PREDICTIVE OF CD34+ ENUMERATION PRIOR TO PERIPHERAL BLOOD STEM CELLS HARVESTING.BALI MEDICAL JOURNAL, [S.l.], v. 3, n. 3, sep. 2014. ISSN 2302-2914. Available at: https://jurnal.harianregional.com/bmj/id-21604. Date accessed: 28 Aug. 2025.

Citation Format

ABNT, APA, BibTeX, CBE, EndNote - EndNote format (Macintosh & Windows), MLA, ProCite - RIS format (Macintosh & Windows), RefWorks, Reference Manager - RIS format (Windows only), Turabian

Issue

Vol 3 No 3 (2014)

Section

Articles

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License