Advances in Tropical Biodiversity and Environmental Sciences 2(1): 18-21, Pebruary 2018                   ISSN: 26220628

DOI: 10.24843/atbes.v02.i01.p05               Available online at: https://ojs.unud.ac.id/index.php/ATBES/article/view/54334

18

Gastric Histopathology of White Rats After Administration of Kedondong Leaves Extract

I Wayan Sudira, I Made Merdana* and Ketut Budiasa

Veterinary Pharmacology and Pharmacy Laboratory, Faculty of Veterinary Medicine, Udayana University

Jl. P.B. Sudirman, Denpasar, Bali, Indonesia

*Corresponding author : [email protected]

Abstract. This study aims to determine the effect of ethanol extract of Kedondong leaves (Spondias dulcis G. Forst) on the gastropathological histology of white rats (Mus musculus). 25 healthy male white rats with weights 200-250 g were randomly divided into five groups. A control group was only given a placebo. The treatment group of kedondong leaf ethanol extract sequentially with a dose of 125, 250, 500 and 1,000 mg/kg/day for 21 days orally. On the 22nd day, all of the animals were sacrificed for the gastric collection, followed by processing of histopathological preparations with Hematoxylin Eosin (HE) staining. The results of the study are all treatment groups were found gastric histology changes in the form of mild inflammatory cell infiltration, mild fatty degeneration and mild necrosis. The number of mice that experienced histopathological changes are different in each group. It can be concluded that the oral administration of kedondong leaf ethanol extract with a range at a dose of 125-1,000 mg/kg/d for 21 days does not cause significant histopathological structure disturbance in the stomach of male white rats.

Keywords: kedondong leaves extract; gastic; histopathological; white rat

  • I.    INTRODUCTION

Kedondong plants (Spondias dulcis, G. Frost) are reported contained chemical compounds such as saponins, flavonoids, and tannins. These compounds are distributed in the fruit, leaves, bark and root bark. The use of traditional medicine is now increasingly popular because it is safer than synthetic chemical drugs and also more economical.

The medicines that enter the body generally will experience absorption, distribution and binding of work targets to cause effects. With or without biotransformation, the drug will then be excreted from the body (Bustami 1995, Muschler). Oral administration will involve various organs of the digestive tract, one of which is the absorption process in the gastric. The stomach is a part of the digestive tract that can dilate, the structure resembles a sac that functions to pulverize food in the digestive process which is assisted by hydrochloric acid (HCl) and enzymes such as pepsin, renin, lipase and paracrine hormone.

The use of traditional medicine as a support for modern medicine that is provided with preparations that are easier to use. In some instances, the content of active ingredients in herbal plants is suspected to be toxic, so that the right dose is needed in its use. As a preventive measure to avoid the occurrence of toxicity, further research needs to be done. In current research,, a study was conducted to determine the effect of the ethanol

extract of kedondong leaf on the gastric histopathological change of the white rats.

  • II.    RESEARCH METHOD

Preparation of kedondong leaves extract

Fresh dried kedondong leaves are blended to powder. The powder was weighed 500 g and soaked with 90% ethanol as much as 1.5 L (ratio of 1: 3), and mixed with a shaker for 3 hours. The mixture is allowed to stand for 72 hours, and then filtered. the supernatant was evaporated with a rotary evaporator and ethanol extract from kedondong leaves was obtained. The extract was stored in the refrigerator at -20ºC before use.

Research procedure and animals development

The study was carried out in the Veterinary Pharmacology and Pharmacy Laboratory of the Faculty of Veterinary Medicine, Udayana University. The experimental animal has followed the protocol and received approval by the Ethical Commission for the Use Animals in Research and Education.

The treatment of kedondong leaf extract with various dosage levels is given for 21 days orally. P0 as a control was only given a placebo. Each treatment group was given a dose of ethanol extract of kedondong leaves as follows P1 = 125 mg/kg/d, P2 = 250 mg/kg/d, P3 = 500 mg/kg/d and P4 = 1,000 mg/kg/d. On the 22nd day all animals were sacrificed and gastric organs were taken for histopathological studies.

Histopathological examination

Histopathological preparation of sample was carried out according to the Kiernan method (2001). The gastric organs were taken and stored in NBF 10%, then the samples are processed histopathological preparations and stained by haematoxyline and eosine stain. Histopathological observation using a light microscope with magnification of 400x, each in 5 microscopic field of view. The observed changes were inflammation cell infiltration, fatty degeneration, and necrosis. Assessment of histopathological changes was done by skoring namely; score 0 - no change, score 1 - if there are mild changes (focal), score 2 - if there is a moderate change (multifocal) and score 3 - if there is a heavy change (diffuse).

Statistic analisys

Histopathological score analysis using the Kruskall Wallis followed by Mann-Whitney test if there were differences found among groups.

  • III.    RESULTS AND DISCCUSION

Result

Histopathological examination results of white rats gastric showing the normal structure in control groups (Fig. 1). Gastric histopathological changes are observed included inflammatory cell infiltration and hydrophic degeneration (Fig. 2 and Fig. 3). features include inflammatory cell infiltration, hydropic and fatty degeneration and necrosis found in groups of animals given extracts at a doses 500 and 1000 mg/kg (Fig. 4 and Fig. 5). Tabulation of the results of histopathological examination is presented in Table 1.

The results of Kruskal-Wallis statistical analysis showed that giving various doses of ethanol extract of Kedondong leaves had no significant effect (P> 0.05) on degeneration, inflammatory cell infiltration and necrosis.

TABEL 1

THE NUMBER OF EXPERIMENTAL ANIMALS UNDERGOING GASTRIC HISTOPALOGICAL CHANGES

Group

Inflammatory Cell Infiltration

Degeneration

Necrosis

0      1       2      3

0

1       2

3

0

1       2

3

P0 (control)

----

-

-          -

-

-

-          -

-

P1 (125 mg/kg)

-          1          -          -

-

1          -

-

-

-          -

-

P2 (250 mg/kg)

-          1          -          -

-

2       -

-

-

-          -

-

P3 (500 mg/kg)

-        2         -         -

-

2       -

-

-

1          -

-

P4 (1.000 mg/kg)

-         3         -         -

-

2       -

-

-

2       -

-


Fig. 1. The histological structure of the gastric is normal in the control group


Fig. 2. Gastric histopathology of P 1 groups (125 mg/kg), found changes: a) infiltration of inflammatory cells, and b) hydropic degeneration


Fig. 3. Gastric histopathology of P 1 groups (250 mg/kg), found changes: a) infiltration of inflammatory cells, and b) hydropic degeneration

Fig. 4. Gastric histopathology of P 1 groups (500 mg/kg), found changes: a) infiltration of inflammatory cells, b) hydropic degeneration, and c) necrosis

Fig. 5. Gastric histopathology of P1groups (1,000 mg/kg), found changes: a) infiltration of inflammatory cells, b) hydropic degeneration, and c) necrosis

Discussion

Kedondong plants are used as herbal medicines because they contain chemical compounds in the form of flavonoids, saponins and tannins. These active compounds contained in other plants are reported to have antioxidant, antibacterial, antiinflamatory, anticancer and immunostimulatory activities. Flavonoids found in all parts of plants such as leaves, roots, wood, skin, fruit and seeds. The mechanism of action of flavonoids in suspected by activating enzymes in the stomach to eliminate mutagen compounds and carcinogens (Markham, 1998). In pharmacokinetics, there may be some active ingredients from herbal contents that are potentially

toxic to the body when consumed, and will first be accommodated in the stomach.

Histologically the stomach consists of four layers, the mucosal layer which is the barrier layer between the body and various materials, including food, as well as toxins and microorganisms that enter through the digestive tract (Bloom and Fawcet, 2004). The first barrier component of the gastric mucosal defense is goblet cells that secrete mucus. The mucus has the ability to facilitate the gliding of food along the digestive tract and prevent epithelial chemical damage. Mucus secretion can also protect the gastric mucosa from foreign agents, such as microorganisms, worms, acidic substances and others (Guyton and Hall, 1997). With enzymatic mechanisms and selection in the digestive system, giving ethanol extract of kedondong leaves on histopathological examination found no toxic in the gastric.

Microscopic examination of gastric histopathology of white rats were found to experience fatty degeneration and hydropic degeneration at all animals group. were degenerated. Fat degeneration is usually characterized by vacuoles of varying magnitude and in severe cases pushing the nucleus to the edge. Fat in the cytoplasm of the pat cell forces the cell nucleus to the edges that appear on microscopic examination. The cells with hydrophic degeneration are usually characterized by enlarged cytoplasm which can be accompanied by the presence of empty spaces (vacuoles) with the cell nucleus remaining in the middle. An enlargement of the cell causes the cells to look tight. Hydrophic degeneration usually occurs mostly in epithelial cells. The cause, among others, due to diet or toxic substances so that lipoproteins are not formed (Himawan, 1992). Degeneration can occur physiologically depending on food and gastric metabolic activity. From various causes, the presence of toxins that enter the body of white rats in large numbers, is a major cause of degeneration in this study.

Giving ethanol extract of kedondong leaves in all animal groups was observed to experience inflammatory cell infiltration except on placebo. Inflammation of inflammatory cells was observed as follows: each of the one samples at a dose of 125 mg/kg, and a dose of 250 mg/kg. Two sample at a treatment dose of 500 mg/kg had inflammatory cell infiltration. At the group at a dose of 1,000 mg/kg, two out of five white rat gastric samples were found have inflammatory cell infiltration. Infiltration of inflammatory cells can occur if cell were damaged to the fundus and pylorus stimulates the release of inflammatory mediators. The series of tasks of the inflammatory mediator begins with acute inflammation and ends with healing.

Histopathological observation of rat's stomach showed a mild necrosis at a dose of 500 mg/kg as much as a rat.

Two mice experienced mild necrosis after being given at a dose of 1,000 mg/kg. Necrosis is characterized by swelling of cells due to the efforts of the plasma membrane to regulate the mechanism of the entry and exit of ions and water. Necrosis involves a large group of cells in the tissue and produces pre-inflammatory molecules that will be infiltrated by inflammatory cells.

The cytoplasm of necrotic cells will look more acidophilic due to denaturation of cytoplasmic protein and lysosome damage. The core chromatin is lumpy, the nucleus shrinks and coloured blue, known as the picnosis process. According to Malik (1992), the gastric mucosa is a barrier between the body and various substances that enter the digestive tract. Therefore, the stomach has a protective system in layers and is very effective for maintaining the integrity of the gastric mucos . Any damage to the gastric mucosa can be repaired by accelerating the replacement of damaged cells. Epithelial cells of the digestive tract continually change and regenerate every 1-3 days. With an enzymatic mechanism and selection in the digestive system, the extract from the Kedondong plant does not cause significant structural damage to the epithelial cells and gastric glands of male white rats. Therefore, this study can recommend that the non-toxic kedondong leaves reach a dose of 1000 mg/kg/d orally in white rats gastric.

  • IV.    CONCLUSION

In this study, it can be concluded that the administration of ethanol extract of kedondong leaves (spondias dulcis, g. forst) with at a dose of 125-1,000 mg/kg/d for 21 days has no significant effect on the histopathological structure of the white rat's gastric.

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