Advances in Tropical Biodiversity and Environmental Sciences 4(1): 1-4, February 2020                   ISSN: 26220628

DOI: 10.24843/ATBES.2020.v04.i01.p01    Available online at: https://ojs.unud.ac.id/index.php/ATBES/article/view/54139

1

Reducing Total Dissolved Solid of Livestock Wastewater with Moringa Seed Powder

I Made Merdana1*, I Ketut Suada2, I Putu Agus Santika Putra3

1Laboratory of Veterinary Pharmacy and Pharmacology, Faculty of Veterinary Medicine, Udayana University 2Laboratory of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University 3Student of Veterinary Internship Program, Faculty of Veterinary Medicine, Udayana University

Jl. P.B. Sudirman, Denpasar, Bali, Indonesia

*Corresponding author: imade_merdana@unud.ac.id

Abstract. In recent years environmental pollution has become a problem that needs attention because it causes negative impacts on ecosystems. The increase in cattle raising is directly proportional to livestock waste produced both from feces and excreted urine. Most people do not know how to process animal wastewater, so they dispose the livestock waste into water areas such as rivers that flow close to settlements. Water quality can be measured by several parameters, one of which is total dissolved solids (TDS). This parameter states the amount of solute both organic and inorganic in a solution. Moringa seeds have a coagulant effect due to their cationic electronic poly protein content. The purpose of this study was to determine the effect of moringa seeds on reducing TDS of livestock wastewater. The sample of this study was cattle farm wastewater taken from the cages of members of the Walung Mekar farmer group. Moringa seed powder concentrations used in this study were 50 mg/L, 100 mg/L and 150 mg/L. Observation was conducted in various time periods 0 minutes, 20 minutes, 40 minutes, 60 minutes. The TDS calculation method used gravimetric calculations (SNI 06-6989.27-2005). The results of variance indicated that Moringa oleifera powder had a very significant effect on the reduction of TDS levels in livestock wastewater. In summary, the best concentration of Moringa oleifera powder at 150 mg/L and the best deposition time at 60 minutes was able to reduce TDS levels.

Keywords: moringa seeds; livestock; wastewater; total dissolved solid

  • I.    introduction

A good and healthy environment as a niche for living and developing an organism, including humans is very necessary for life. The environment is a good and healthy environment if the organisms in it are able to live and develop normally by the conditions and supporting resources [1]. Water quality can be measured by several parameters, one of which is Total Dissolved Solid (TDS). This parameter states the amount of solute both organic and inorganic in a solution. This parameter is used to determine whether a water source is suitable to be used as a source of drinking water [2]. Based on the regulation of the Indonesia minister of environment, the maximum level of TDS for clean water quality is 2.000 mg/L [3].

Moringa olefra seeds contain 4-(α-L-rhamnpyranosyloxy) benzyl isothiocyanate which has been identified as having antimicrobial activity. This component is a bacteriostatic compound which means it can inhibit bacterial growth or metabolic activity [4]. In addition to antimicrobial effects, Moringa seeds also have a coagulant effect due to their protein content which is cationic polyelectrolytes [5]. This means that the protein in

Moringa seeds can be used to purify water, the protein contains amino acids, and if dissolved in water will undergo ionization or dissociation [6].

  • II.    research method

Object of research

The sample used was cattle livestock wastewater taken from the cages of members of the Walung Mekar farmer group, Mr. Subaga, in Serongga Village. It has the wastewater was usually dumped directly into the river without any prior processing.

Research procedure

Moringa seeds that are ripe or old are peeled from their shells and the seeds are taken and then dried in an oven at 105oC for 30 minutes to reduce the water content. Moringa seeds that have been dried, then crushed and sieved with a 60 mesh siever in order to get the fine Moringa seed powder. As much as 1 gram of Moringa seed powder was then dissolved with 200 ml of distilled water so that a concentration of 5000 mg/L was obtained.

Moringa seed powder concentrations used in this study were 50 mg/L, 100 mg/L and 150 mg/L. This

determination of concentration is a modification of the study of Wibawarto [7]. Moringa seeds effectively improve the quality of domestic waste at a concentration of 40 mg/L. The concentration used in this study was higher due to the characteristics of cattle farm waste such as turbidity, odor, acidity as well as organic and inorganic content different when compared to domestic waste.

Wastewater is prepared in each beaker glass of 500 ml, followed by coagulation process with Moringa seeds with a concentration of P0= 0 mg/L (control), P1= 50 mg/L, P2= 100 mg/L, P3= 150 mg/L. Each sample was stirred quickly for 2 minutes at a stirring speed of 200 rpm and continued with slow stirring for 5 minutes at a speed of 60 rpm. After the coagulation process is complete, the solution is allowed to stand with a variation of time 0 minutes, 20 minutes, 40 minutes, 60 minutes.

Total Dissolved Solid (TDS) Test

Samples that had been coagulated with Moringa seed powder associated with each coagulation time, taken with a volume pipette of 50 ml to 100 ml, and put into a filter that has been equipped with a vacuum pump and filter paper. After the filtered sample all the filter paper was rinsed 3 times with 10 ml distilled water each, then continue suctioning for about 3 minutes. After perfect filtering, the entire filter was transferred to a cup that has a fixed weight. The filter in the cup was evaporated to dryness over a water bath. A cup containing dissolved solids that have dried was put into the oven at 180ºC for about 1 hour. Then the cup was removed from the oven with tongs and chill it in a desiccator. When the cup cooled immediately weigh it with an analytical balance. The heating process in the oven was repeated to obtain a fixed weight. According to gravimetric calculations (SNI: 06-6989.27-2005) to get the TDS estimate as follows:

TDS (mg/L) =


(W1-W0) x 106

Volume sample test

Note: W0 = weight remains empty cup after heating 180oC, W1 = fixed weight of the cup containing TDS after 180ºC heating

Data analysis

To find out the effect of Moringa seed powder concentration and the time of deposition time on the decrease in TDS, analyzed using ANOVA. If there are significant differences, it will be followed by Duncan's multiple range test [8].

  • III.    result and discussion

Result

Total Dissolved Solid is dissolved solids contained in water. The data results of TDS measurement from cattle

wastewater added to the concentration of Moringa seed powder can be seen in Table 1 and Table 2.

TABLE I

DUNCAN TEST RESULT OF TDS LEVEL OF LIVESTOCK WASTEWATER BY CONCENTRATION OF MORINGA POWDER

Concentration of Moringa (mg/L)

Mean ± SD

Sig. 0.05

0

2.620,0 ± 107,28

a

50

2.550,8 ± 97,18

b

100

2.510,0 ± 104,62

c

150

2.536,4 ± 100,22

d

Note: Values with different letters to the column show significantly different (P<0.05)

TABLE II

DUNCAN TEST RESULT OF TDS LEVEL OF LIVESTOCK WASTEWATER BY TIME OF SIDEMENTATION

Time to Sedimentation (minute)

Mean ± SD

Sig. 0.05

0

2 572,5 ± 98,82

a

20

2.540,8 ±

111,47

2 529,1 ±

116,57

2 503,3 ±

132,82

b

40

b

60

c

Note: Values with different letters to the column show significantly different (P<0.05), otherwise values with the same letter indicate no significant difference (P> 0.05).

Discussion

The results of this study showed a decrease in TDS levels, but still above the standard quality of cattle farm waste according to Minister of Environment regulation number 5 of 2014 [3], namely the maximum quality standard of TDS 2.000 mg/L. This is caused by the large amount of organic and inorganic substances contained in cow livestock waste, so that the TDS value was higher than quality standard. Further processing such as filtration is needed.

Effect of Moringa powder concentration on TDS

The results of variance proved that the concentration of Moringa seed powder has a very significant effect (P<0.01) on TDS of cattle farm waste. Duncan test results showed concentrations of 0 mg/L with 50 mg/L, significantly different (P<0.05), 0 mg/L with 100 mg/L significantly different (P<0.05) and 0 mg/L with 150 significantly different (P<0.05). Concentrations of 50 mg/L with 100 mg/L were significantly different (P<0.05) and 100 mg/L with 150 mg/L were significantly different (P<0.05). Moringa seed concentration used in this study was able to reduce the TDS value of cattle farm waste. As the concentration used in the study of Januardi and

Mukarlina [9], the lowest concentration of Moringa seed powder was 66.7 mg/L was able to provide good results to reduce TDS levels. Decreased levels of TDS occur because Moringa seed powder has a positively charged protein that will bind the negative charges on liquid waste. This statement is strengthened from the results of research by Mubiyi and Evison [10], stating that the decrease in turbidity in waste is caused by the incorporation of a charge between the positively charged Moringa seed protein and the cause of turbidity with negatively charged water, so that the growing flocks will be deposited [11].

Effect of sedimentation time on TDS

The results of variance proved that each deposition time also has a very significant effect (P<0.01) on the reduction in TDS of cattle farm waste. Duncan test results on the duration of deposition showed that the duration of deposition of the 0 minute to the 20 minute was significantly different (p<0.05), the 0 minute to the 40 minute were significantly different (p<0.05) and the 20 minute to 0 minute with the 60 minute was significantly different (p<0.05). The duration of the deposition of the 20 minute to the 40 minute was not significantly different (p>0.05) and the 40th minute to the 60th minute was significantly different. This was confirmed by the study of [12-13] deposition time is the time required to precipitate flocks that have formed in the coagulation process, the cationic protein in the moringa seeds will experience van der Waals force. The van der Waals force is a relatively weak force that works between molecules. To decide the force requires energy around 0.4-40 Id/mol [12-14].

The energy needed to break the van der Waals force is very low or easy to break, if left for a long time the bonds that have formed can be broken down again and TDS levels will increase [15-16]. In this study, for a long time, the deposition of 60 minutes was still effective.

  • IV.    CONCLUSION

Moringa seed powder at concentration of 50 mg/L, 100 mg/L, 150 mg/L and the duration of deposition of 20 minutes, 40 minutes, 60 minutes can decreased TDS levels. Moringa seed powder concentration of 150 mg/L and 60 minutes of deposition time had an optimal effect on TDS levels.

ACKNOWLEDGMENT

The researcher thanks so much for the farmer group Walung Mekar, Gianyar, and to all those who have helped in the completion of this journal article.

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