Authors:

BARAELA EZRA WIJAYA, RINDANG DWIYANI, NI NYOMAN ARI MAYADEWI

Abstract:

“Callus Induction and Multiplication of Eucalyptus sp. on Various Media Callus Induction Medium (CIM) In Vitro. Callus induction in vitro can be done by planting specific part of plant such as leaf, root, stem, etc in aseptic condition by using medium that contains 2,4-D, a plat regulator for promoting callus formation. One of the objectives of callus production in vitro is to form secondary metabolites, because eucalyptus is known as a producer of essential oils. This research is an initial study for in vitro production of secondary metabolites for further research. The aim of this study was to determine the most suitable callus induction medium for callus formation and callus multiplication for Eucalyptus grown in vitro. This research was designed as a Completely Randomized Design (CRD), five treatments, repeated five times. Woody Plant Medium (WPM) was used as the basic media. Several Callus induction Medium (CIM) were used as treatments, i.e. CIM1 = 1 ppm 2,4-D; CIM2 = 1,5 ppm 2,4-D; CIM3 = 2 ppm 2,4-D; CIM4 = 1 ppm NAA+1 ppm BAP; and CIM5 = 2 ppm NAA+ 2 ppm BAP. Variables observed were the time of curly leaf formation, time of the emerging callus, colour and texture of the callus, callus’s weight, callus surface area, the weight of callus after multiplication. The result showed that CIM4 and CIM5 had the fastest emerging callus (7 days after planting), CIM1 and CIM2 formed green and crumb texture of callus, CIM1 produced the highest of callus’s weight, and CIM2 resulted in the highest of callus’s surface area. In conclusion, the most suitable CIM for callus induction was CIM1, while for callus multiplication was CM2.”

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References

  • Alvarez, M.A. (2014). Plant Biotechnology for Health: From Secondary Metabolites to Molecular Farming. Springer International Publishing, Switzerland.
  • Ardiana, D.W. (2009). Teknik Pemberian Benzyl Amino Purin untuk Memacu Pertumbuhan Kalus dan Tunas pada Kotiledon Melon (Cucumis melo L.). Buletin Teknik Pertanian, 14 (2): 5053.
  • Astiani, D.P., Jayuska, A., & Arreneuz, S. (2014). Uji Aktivitas Antibakteri Minyak Atsiri Eucalyptus pellita Terhadap Bakteri Escherichia coli dan Staphylococcus aureus. JKK, 3 (3): 49-53.
  • Bustami, M.U. 2(011). Penggunaan 2,4-D Untuk Induksi Kalus Kacang Tanah. Media Litbang Sulteng, Vol IV: 139-141.
  • Damanik, M. (2009). Kajian Minyak Atsiri pada Eukaliptus (Eucalyptus urophylla) Umur 4 Tahun di PT Toba Pulp Leastari, TBK. Skripsi USU: 11-14.
  • Dwiyani, R. (2012). Micropropagation of an orchid of Vanda tricolor Lindl. suavis var. from Bali carrying KNOTTED1-LIKE Arabi- dopsis thaliana (KNAT1) gene (in Indonesian). Disertation. Postgraduate School. Gadjah Mada University. Yogyakarta.
  • Dwiyani, R. (2015). Kultur Jaringan Tanaman. Palawa Sari, Denpasar.
  • Fitriani, Y. (2019). Teknik Sterilisasi dan Efektivitas 2,4-D terhadap Pembentukan Kalus Eksplan Daun Nilam (Pogostemon Cablin Benth) In Vitro. Journal of Agricultural Science and Biotechnology, 8 (1):1-7
  • Fitriani, Y., Wijana, G., & Darmawati, I.A.P. (2019). Teknik Sterilisasi dan Efektivitas 2,4-D Terhadap Pembentukan Kalus Eksplan Daun Nilam (Pogostemon cablin Benth). In vitro. J. Agric. Sci. and Biotechnol : 42-51.
  • George, E.F., & Sherrington, P.D. (1984). Plant Propagation by Tissue Culture. Exegetics Limited, England.
  • Hartmann, D., Burton, W.B., & Kerr, F. (1997). Atlas of Galactic Neutral Hydrogen.
  • Lestari, E.G. (2011). Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman Melalui Kultur Jaringan. Jurnal Agro-Biogen. 7(1), 63-68.
  • Mahadi, I., Syafi’i, W., & Sari, Y. (2016). Induksi Kalus Jeruk Kasturi (Citrus microcarpa) Menggunakan Hormon. Jurnal Ilmu Pertanian Indonesia (JIPI), Vol. 21 No. 2: 86
  • Mindawati, N., Indrawan, A., Mansur, I., & Rusdiana, O. (2010). Kajian Pertumbuhan Tegakan Hybrid Eucalyptus urogandis di Sumatera Utara. Jurnal Penelitian Hutan Tanaman, 7(1): 39- 50.
  • Palupi, A.D., Solichatun, & Marliana, S.D. (2004). Pengaruh Asam 2,4- Diklorofenoksiasetat (2,4-D) dan Benziladenin (BA) terhadap Kandungan Minyak Atsiri Kalus Daun Nilam (Pogostemon cablin Benth.). BioSMART 6(2): 99-103.
  • Purba, M.P. (2009). Besar Aliran Permukaan (Run-off) Pada Berbagai Tipe Kelerengan Dibawah Tegakan Eucalyptus sp. Skripsi. Universitas Sumatra Utara.
  • Ribkahwati (2015). Profil Minyak Atsiri Kalus Daun Pucuk Mawar (Rosa hybrid L. varietas hybride tea) Lokal Batu Hasil Elisitasi dengan Cahaya. Disertasi, Program Studi S3 MIPA, Fakultas Sains dan Teknologi, Universitas Airlangga.
  • Sari, N., Suwarsi, E., & Sumadi. (2014). Optimasi Jenis dan Konsentrasi ZPT dalam Induksi Kalus Embriogenik dan Regenerasi menjadi Planlet pada Carica pubescens (Lenne & K.Koch). Biosaintifika, 6 (1): 56
  • Setianingrum, A. (2010). Kajian Penggunaan Berbagai Konsentrasi BAP dan 2,4-D Terhadap Induksi Kalus Jarak Pagar (Jatropha curcas L.) Secara In vitro. Skripsi. Fakultas Pertanian Universitas Sebelas Maret.
  • Sulichantini, E.D. (2016). Pertumbuhan Tanaman Eucalyptus pellita F. Muell Di Lapangan Dengan Menggunakan Bibit Hasil Perbanyakan Dengan Metode Kultur Jaringan Stek Pucuk, dan Biji. ZIRAA’AH, Vol 41, no 2: 269-275.
  • Yusnita. (2003). Kultur Jaringan Cara Memperbanyak Tanaman Secara Efisien. AgroMedia Pustaka, Jakarta

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https://jurnal.harianregional.com/agrotrop/full-96910

Published

2023-01-17

How To Cite

WIJAYA, BARAELA EZRA; DWIYANI, RINDANG; MAYADEWI, NI NYOMAN ARI. Induksi dan Multiplikasi Kalus Eucalyptus sp. pada Berbagai Media Callus Induction Medium (CIM) Secara In Vitro.Agrotrop : Journal on Agriculture Science, [S.l.], v. 13, n. 1, p. 76-84, jan. 2023. ISSN 2654-4008. Available at: https://jurnal.harianregional.com/agrotrop/id-96910. Date accessed: 28 Aug. 2025. doi:https://doi.org/10.24843/AJoAS.2023.v13.i01.p07.

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Issue

Vol 13 No 1 (2023)

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Articles

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