Authors:

Ketut Widyani Astuti, Ni Putu Ayu Dewi Wijayanti, I Gusti Ngurah Agung Dewantara Putra, Ni Putu Linda Laksmiani

Abstract:

“Kappaphycus alvarezii Doty is a species of red algae ( Rhodophyceae) producing kappa carrageenan. The value of carrageenan will increase if it is able to form thermoreversible gels. The quality of carrageenan is determined by the isolation method which includes several stages, namely soaking, extraction, purification, and drying. This study aims to obtain an optimum isolation method of carrageenan from seaweed Kappaphycus alvarezii Doty using the factorial experimental design software, Design Expert Version.7.0.0. with the characteristics that meet the standards set by FAO. The seaweed was collected from the sea in Nusa Lembongan, Bali Province. The carrageenan isolation method was optimized by varying three factors, namely the concentration of NaOH (4% and 8%), extraction time (0.5 hour and 3 hours), and precipitation time (0.25 hour and 0.5 hour) in order to obtain 8 formulae which then went through an evaluation of the physical and chemical characteristics of the carrageenan. The physical and chemical characteristics include the yield, viscosity, sulfate content, and ash content, analyzed using Design Expert Version 7.0.0 program to determine the optimum method of isolation. The analysis showed that the optimum method of carrageenan isolation was using NaOH with a concentration of 4.40%, the extraction time of 2.16 hours and the precipitation time of 0.29 hour. The physical and chemical characteristics of carrageenan with the optimum isolation method include the yield of 25.73%, the viscosity of 11.92 cPs, sulfate content of 31.95% and the ash content of 26.16% which have met the standards set by FAO.”

Keywords

Keyword Not Available

Downloads:

Download data is not yet available.

References

  • [1] Hellebust, J. A., J. S. Cragie. 1978. Handbook of Phycological
  • Methods. London: Cambridge University Press.
  • [2] Rowe, R.C., P. J. Sheskey, and J. W. Paul. 2009. Handbook of
  • Pharmaceutical Exipients. Sixth Edition. London: Pharmaceutical
  • Press.
  • [3] Desiana, E., and T. Y. Hendrawati. 2015. Seminar Nasional Sains
  • dan Teknologi: Pembuatan Karagenan dari Eucheuma cottonii
  • dengn Ekstraksi KOH Mengggunakan Variabel Waktu Ekstraksi.
  • ISSN: 2407-1846.
  • [4] Anggadiredja, J., Z. Achmad., P. Heri, and S. Istani. 2006. Rumput
  • Laut: Pembudidayaan, Pengolahan dan Pemasaran Komoditas
  • Perikanan Potensial. Jakarta: Penebar Swadaya.
  • [5] Distantina, S., Fadilah, Y. C. Danarto, Wiratni, and M. Fahrurrozi.
    1. Efek Bahan Kimia pada Tahap Presipitasi terhadap Rendemen
  • dan Sifat Karagenan dari Rumput laut Eucheuma cottoni.
  • Ekuilibrium. Vol. 8, No. 2:47-53.
  • [6] Mtolera, M.S., and Buriyo, A.S., 2004, Studies on Tanzanian
  • Hypneaceae: Seasonal Variation in Content and Quality of Kappa-
  • Carrageenan from Hynea musciformis, Western Indian Ocean J.
  • Mar. Sci., 3, pp. 43-49
  • [7] Pelegrin, Y.F., Robledo, D., and Azamar, J.A.,2006, Carrageenan of
  • Eucheuma isiforme (Solieriaceae, Rhodophyta) from Yucatanm
  • Mexico. I. Effect of Extraction Conditions, Botanica Marina, 49, pp.
  • 65-71.
  • [8] AOAC. 1995. Official Methods of Analysis of the Association of
  • Official Analytical Chemist. Virginia: AOAC Inc. Arlington.
  • [9] Andriani D. 2006. Pengolahan Rumput Laut (Eucheuma cottonii)
  • Menjadi Tepung ATC (Alkali Treated Cottonii) dengan Jenis dan
  • Konsentrasi Larutan Alkali yang Berbeda. (undergraduate thesis).
  • Makassar: Faculty of Agriculture and Forestry, Universitas
  • Hasanudin.
  • [10] Distantina, S., Fadhilah, Rochmadi, Wiratni, M. Fahrurozi. 2010.
  • Proses Ekstraksi Karagenan dari Eucheuma cottonii. Seminar
  • Rekayasa Kimia dan Proses. Department E. H. Miller, “A note on
  • reflector arrays (Periodical style—Accepted for publication),” IEEE
  • Trans. Antennas Propagat., to be published.
  • [11] Syamsuar. 2006. Karakteristik Karaginan Rumput Laut Eucheuma
  • Cottonii pada Berbagai Umur Panen, Konsentrasi KOH dan Lama
  • Ekstraksi. (Undergraduate thesis). Bogor: Graduate School of IPB.
  • [12] Wang, W. C. 1995. Ohmic Heating of Foods: Physical Properties
  • and Applications. (Dissertation). Colombus: The Ohio State
  • University.
  • [13] Ary, A. P. 2015. Studi Pengaruh Metode Presipitasi terhadap
  • Rendemen yang dihasilkan dari Proses Ekstraksi Rumput Laut
  • Eucheuma cottonii Melalui Pemanasan OHMIC. (Skripsi).
  • Makassar: Universitas Hassanudin.
  • [14] Moirano, A. L. 1977. Sulfated Seaweed Polysacharides in Food
  • Colloids. Westport, Connecticut: The AVI Publishing Company.
  • [15] Ningsih, F. L. 2014. Jenis dan Konsentrasi Alkali dengan Presipitasi
  • KCl yang Berbeda terhadap Mutu Karaginan dari Rumput Laut
  • Kappaphycus alvarezii Asal Pulo Panjang Serang Banten.
  • (Undergraduate thesis). Banten: Universitas Sultan Ageng Tirtayasa.
  • [16] Romenda, A. P., R. Pramesti, A. B. Susanto. 2013. Pengaruh
  • Perbedaan Jenis dan Konsentrasi Larutan Alkali terhadap Kekuatan
  • Gel dan Viskositas Karaginan Kappaphycus alvarezii Doty. Journal
  • of Marine Research. Vol. 2, No. 1:127-133
  • [17] Food and Agriculture Organization (FAO). 2007. Carrageenan. FAO
  • JECFA Monogaphs 4
  • [18] Winarno. F. G. 1990. Kimia Pangan dan Gizi. Jakarta: Gramedia
  • Pustaka Utama.
  • [19] Winarno, F. G., S. Fardiaz, and D. Fardiaz. 1996. Teknologi
  • Pengolahan Rumput Laut. Jakarta: Putaka Sinar Harapan.

PDF:

https://jurnal.harianregional.com/jhsm/full-36977

Published

2018-01-25

How To Cite

ASTUTI, Ketut Widyani et al. Optimization Of Isolation Method Of Carrageenan From Kappaphycuss Alvarezii Doty Using Factorial Experimental Design.Journal of Health Sciences and Medicine, [S.l.], v. 1, n. 2, p. 4-7, jan. 2018. ISSN 2622-0555. Available at: https://ojs.unud.ac.id/index.php/JHSM/article/view/36977. Date accessed: 28 Aug. 2025.

Citation Format

ABNT, APA, BibTeX, CBE, EndNote - EndNote format (Macintosh & Windows), MLA, ProCite - RIS format (Macintosh & Windows), RefWorks, Reference Manager - RIS format (Windows only), Turabian

Issue

Vol 1 No 2 (2017): JHSM (September 2017)

Section

Articles

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License